Fig. 5. Detection of Caspase-3, Caspase-10 and Bcl-2 mRNA transcripts in PDT-treated HeLa cells. Total RNA was isolated from HeLa cells for RT-PCR analysis using the Caspase-3, Caspase-10 and Bcl-2 gene-specific primers. The Beta-actin gene was used as a control. Line 1-4, 5-8 and 9-10 all represent 0, 15, 30 and 60 pg/mL R-PE PDT-treated HeLa cells.