Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 3, Issue of July 15, 2008
 

Table 1. RAPD primers used to detect polymorphism, number of bands for polymorphism between landraces per primer.


RAPD Primer

Sequence (5’-3’)

Number of bands

Number of polymorphic markers

Percentage
polymorphism

PIC value

OPC9

CTCACCGTCC

2

2

100.00

0.353

OPC10

TGTCTGGGTG

8

4

50.00

0.283

OPC11

AAAGCTGCGG

8

3

37.50

0.122

OPC12

TGTCATCCCC

20

10

50.00

0.184

OPC13

AAGCCTCGTC

8

5

62.50

0.253

OPC14

TGCGTGCTTG

11

2

18.18

0.045

OPC15

GACGGATCAG

8

6

75.00

0.313

OPC16

CACACTCCAG

9

6

66.67

0.255

OPC17

TTCCCCCCAG

14

8

57.14

0.162

OPC18

TGAGTGGGTG

8

5

62.50

0.240

OPC19

GTTGCCAGCC

14

7

50.00

0.146

OPC20

ACTTCGCCAC

16

7

43.75

0.137

OPH12

ACGCGCATGT

10

7

70.00

0.242

OPH14

ACCAGGTTGG

17

16

94.12

0.287

OPH17

CACTCTCCTC

2

0

0.00

0.180

OPH19

CTGACCAG.CC

10

9

90.00

0.404

OPH20

GG.GAGACATC

16

3

18.75

0.053

OPM1

GTTGGTGGCT

10

8

80.00

0.228

OPM2

ACAACGCCTC

14

10

71.43

0.242

OPM4

GGCGGTTGTC

8

6

75.00

0.323

OPM5

GGGAACGTGT

5

4

80.00

0.396

OPM6

CTGGGCAACT

17

16

94.12

0.300

OPM7

CCGTGACTCA

7

7

100.00

0.254

OPM8

TCTGTTCCCC

16

16

100.00

0.436

OPM9

GTCTTGCGGA

24

24

100.00

0.283

OPM10

TCTGGCGCAC

15

13

86.67

0.251

OPO2

ACGTAGCGTC

22

19

86.36

0.306

OPO3

CTGTTGCTAC

15

15

100.00

0.369

OPO4

AAGTCCGCTC

18

16

88.89

0.354

OPO5

CCCAGTCACT

11

7

63.64

0.167

OPO8

CCTCCAGTGT

7

5

71.43

0.240

OPO10

TCCCACGCAA

31

31

100.00

0.269

OPO11

CCTCCAGTGT

7

6

85.71

0.333

OPO13

GACAGGAGGT

12

12

100.00

0.305

OPQ1

GGGACGATGG

17

4

23.53

0.082

OPQ2

TCTGTCGGTC

14

13

92.86

0.354

OPQ3

GGTCACCTCA

14

10

71.43

0.169

OPQ4

AGTGCGCTGA

17

13

76.47

0.193

OPQ5

CCGCGTCTTG

14

11

78.57

0.227

OPQ6

GAGCGCCTTG

15

12

80.00

0.189

OPQ7

CCCCGATGGT

9

8

88.89

0.263

OPQ9

GGCTAACCGA

22

19

86.36

0.223

OPQ10

TGTGCCCGAA

12

7

58.33

0.193

OPY9

GCAGCGCAC

14

10

71.43

0.239

OPZ1

TCTGTGCCAC

13

8

61.54

0.118

OPZ05

TCCCATGCTG

12

10

83.33

0.246

OPZ08

TCCCATGCTG

14

9

64.29

0.203

OPZ11

CTCAGTCGCA

16

15

93.75

0.280

OPZ12

TCAACGGGAC

12

3

25.00

0.065

OPZ13

GACTAAGCCC

1

0

0.00

0.260

OPZ16

TCCCCATCAC

12

6

50.00

0.142

OPBA13

AGGGCGAATG

17

8

47.06

0.266

OPBA15

GAAGACCTGG

13

11

84.62

0.212

OPBA16

CCACGCATCA

17

8

47.06

0.121

OPBB1

ACACTGGCTG

18

7

38.89

0.120

OPBB5

GGGCCGAACA

7

5

71.43

0.317

OPBB6

CTGAAGCTGG

18

15

83.33

0.277

OPBB7

GAAGGCTGGG

18

11

61.11

0.350

OPBB9

AGGCCGGTCA

9

4

44.44

0.122

OPBB11

TGCGGGTTCC

16

11

68.75

0.259

OPBB13

CTTCGGTGTG

14

12

85.71

0.233

OPBB14

GTGGGACCTG

7

5

71.43

0.300

OPBC2

ACAGTAGCGG

15

15

100.00

0.448

OPBC3

GGCTTGACCT

10

8

80.00

0.422

OPBC4

CCACGTGCCA

15

6

40.00

0.149

OPBC10

AACGTCGAGG

16

14

87.50

0.304

OPBC20

AGCACTGGGG

15

15

100.00

0.219

OPBD9

CCACGGTCAG

19

11

57.89

0.180

OPBD10

CTGCTGGGAC

18

17

94.44

0.257

OPBD16

GAACTCCCAG

11

8

72.73

0.273

OPBD17

GTTCGCTCCC

16

12

75.00

0.245

OPBD18

ACGCACACTC

18

18

100.00

0.298

OPBD19

GGTTCCTCTC

14

11

78.57

0.271

OPBD20

AGGCGGCACA

18

14

77.78

0.194

 

Total

987

719

 

 

 

Average per primer

13.35

 

70.30

0.243


  Supported by UNESCO / MIRCEN network
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