Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 3, Issue of July 15, 2008
 

Table 2. ISSR primers used to detect polymorphism, number of bands for polymorphism between landraces per primer.


ISSR Primer

Sequence (5’-3’)

Number of bands

Number of polymorphic markers

Percentage polymorphism 

PIC value

UBC807

AGAGAGAGAGAGAGAGT

16

2

12.50

0.041

UBC808

AGAGAGAGAGAGAGAGC

10

7

70.00

0.260

UBC810

GAGAGAGAGAGAGAGAT

3

2

66.67

0.200

UBC811

GAGAGAGAGAGAGAGAC

6

1

16.67

0.080

UBC814

CTCTCTCTCTCTCTCTA

6

2

33.33

0.160

UBC816

CACACACACACACACAT

11

6

54.55

0.247

UBC820

GTGTGTGTGTGTGTGTC

10

10

100.00

0.296

UBC821

GTGTGTGTGTGTGTGTC

19

12

63.16

0.404

UBC824

TCTCTCTCTCTCTCTCG

8

8

100.00

0.308

UBC826

ACACACACACACACACC

12

3

25.00

0.097

UBC827

ACACACACACACACACG

12

3

25.00

0.045

UBC828

TGTGTGTGTGTGTGTGA

5

0

0.00

0.000

UBC829

TGTGTGTGTGTGTGTGC

13

5

38.46

0.131

UBC834

AGAGAGAGAGAGAGAGYT

26

6

23.08

0.058

UBC836

AGAGAGAGAGAGAGAGYA

8

6

75.00

0.135

UBC840

GAGAGAGAGAGAGAGAYT

16

2

12.50

0.043

UBC841

GAGAGAGAGAGAGAGAYC

16

11

68.75

0.233

UBC843

CTCTCTCTCTCTCTCTRA

18

17

94.44

0.377

UBC844

CTCTCTCTCTCTCTCTRC

17

16

94.12

0.374

UBC846

CACACACACACACACART

12

5

41.67

0.127

UBC849

GTGTGTGTGTGTGTGTYA

10

10

100.00

0.296

UBC851

GTGTGTGTGTGTGTGTYG

5

3

60.00

0.332

UBC852

TCTCTCTCTCTCTCTCRA

4

2

50.00

0.125

UBC853

TCTCTCTCTCTCTCTCRT

9

4

44.44

0.149

UBC854

TCTCTCTCTCTCTCTCRG

20

19

95.00

0.259

UBC855

ACACACACACACACACYT

24

23

95.83

0.253

UBC857

ACACACACACACACACYG

18

13

72.22

0.250

UBC859

TGTGTGTGTGTGTGTGRC

15

13

86.67

0.325

UBC862

AGCAGCAGCAGCAGCAGC

13

12

92.31

0.311

UBC864

ATGATGATGATGATGATG

20

19

95.00

0.287

UBC867

GGCGGCGGCGGCGGCGGC

5

5

100.00

0.264

UBC873

GACAGACAGACAGAC A

9

0

0.00

0.000

UBC880

GGAGAGGAGAGGAGA

23

19

82.61

0.298

UBC884

HBHAGAGAGAGAGAGAG

14

3

21.43

0.059

UBC885

HBHAGAGAGAGAGAGAG

13

7

53.85

0.168

UBC886

VDVCTCTCTCTCTCTCT

18

9

50.00

0.267

UBC895

AGGTCGCGGCCGCNNNNNNAT

15

11

73.33

0.240

 

Total

479

296

 

 

 

Average per primer

12.94

 

61.79

0.203

Single letter abbreviations for mixed base positions
N = (A, G, C, T); R = (A, G); Y = (C, T); B = (C, G, T); (i.e. not A); D = (A, G, T) (i.e. not C); H = (A, C, T) i.e. not G); V = (A, C, G) (i.e. not T).


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