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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Table 4. Inhibition of Vibrio harveyi PSU 2015 by 0.45 µm and 0.22 µm culture filtrates from Pseudomonas sp. W3.


Treatment

Inhibition zone (mm)

0.45 µm culture filtrate

0.22 µm culture filtrate

Enzyme

 

 

 Control

17.8 ± 0.3a

15.2 ± 0.4a

 Proteinase K

13.8 ± 0.2bc

14.7 ± 0.7abc

 Pronase

13.9 ± 0.4bc

14.4 ± 0.5abc

 Pepsin

14.4 ± 0.2bc

14.3 ± 0.2abc

 α-chymotrypsin

13.0 ± 0.2c

14.0 ± 0.5c

 Trypsin

14.0 ± 0.6bc

15.1 ± 0.7ab

 Lysozyme

14.5 ± 0.2bc

14.8 ± 0.2abc

 Lipase

14.8 ± 0.4b

14.4 ± 0.4abc

 α-amylase

15.0 ± 0.4b

14.0 ± 0.4bc

 Temperature of incubation (ºC)

 

 

 Control (30)

18.0 ± 0.5a

15.4 ± 0.4a

 50

17.5 ± 0.5a

15.3 ± 0.3a

 65

16.5 ± 0.4b

14.7 ± 0.1ab

 75

15.9 ± 0.5b

15.0 ± 0.4ab

 100

16.2 ± 0.3b

14.7 ± 0.4ab

 Autoclave (121ºC, 15 min)

15.1 ± 0.4c

14.7 ± 0.2ab

 Autoclave (121ºC, 30 min)

14.3 ± 0.2d

14.0 ± 0.7b

pH

 

 

 Control (pH 7.40)

17.1 ± 0.5a

16.8 ± 0.7a

 1

5.3 ± 0.5f

10.1 ± 0.7c

 2

13.1 ± 0.4cd

15.5 ± 0.7b

 3

13.3 ± 0.5c

15.4 ± 0.5b

 4

13.9 ± 0.5bc

16.1 ± 0.3ab

 5

14.4 ± 0.5bc

15.6 ± 0.8ab

 6

16.5 ± 0.3b

16.0 ± 0.5ab

 7

16.3 ± 0.3b

15.5 ± 0.8ab

 8

12.4 ± 0.4d

15.5 ± 0.3ab

 9

12.2 ± 0.4d

14.9 ± 0.6b

 10

11.1 ± 0.4e

14.9 ± 0.8b

Mean value ± standard deviation of nine observations for enzyme treatments and pH activities; three observations for temperature. Different letters in the same column denote significant differences (p < 0.05).


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