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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 3, Issue of May 15, 2010
 

Table 2. Banding patterns and amplifications of 32 wheat lines using selected 15 RAPD primers.



No

Primer Code

Primer sequence

No. of amplified bands

No. of polymorphic bands

Degree of polymorphism (%)

1

OPA-05

5'-AGGGGTCTTG-3'

10

9

90.0

2

OPA-10

5'-GTGATCGCAG-3'

6

1

16.7

3

OPA-11

5'-CAATAGCCGT-3'

6

2

33.3

4

OPB-01

5'-GTTTCGCTCC-3'

7

5

71.4

5

OPB-04

5'-GGACTGGAGT-3'

4

3

75.0

6

OPB-11

5'-GTAGACCCGT-3'

6

3

50.0

7

OPC-07

5'-GTCCCGACGA-3'

11

4

36.4

8

OPC-10

5'-TGTCTGGGTG-3'

15

6

40.0

9

OPD-08

5'-GTGTGCCCCA-3'

13

11

84.6

10

OPF-15

5'-CCAGTACTCC-3'

8

5

62.5

11

OPJ-01

5'-CCCGGCATAA-3'

11

4

36.4

12

OPK-02

5'-GTCTCCGCAA-3'

8

5

62.5

13

OPK-08

5'-GAACACTGGG-3'

10

9

90.0

14

OPK-12

5'-GTGCAACGTG-3'

11

8

72.7

15

OPK-15

5'-CTCCTGCCAA-3'

14

11

78.6

Overall

140

86

61.4

 


Supported by UNESCO / MIRCEN network