Run No.	Reaction Condition	R1	R2	R3	R4	time	R5	R6
1
	RT=20º C	200	2,72	2,9	0,5	0	376	0
	CE=5x10-7	100	0,83	2,94	1.00	150	88,2	76,5
	Mol/L	100	0,91	3,01	1.00	300	30,27	91,9
11
	RT=35º C	20	0,87	4,19	1.00	60	41,3	0,0a
	CE=5x10-7	70	0,88	2,63	1.00	120	23,4	43,3
	mol/L	100	0,84	4,27	1.00	610	19,7	52,3
35
	RT=35º C	80	9,5	2	1.00	0	380	0
	CE=1,3x10-7	100	1,1	1,6	1.00	30	68,75	81,9
	Mol/L	100	0,7	3,3	1.00	60	21,2	94,4
Where R1 is the size of internal standard, microlitre; R2 is the height of p-cresol which is a height of a peak in HPLC, centimetre; R3 is the height of phenyl standard which is also a height of a peak in HPLC, centimetre; R4 is the size of sample, millilitre; R5 is the relative concentration of unreacted p-cresol; R6 is the conversion of p-cresol, %; time is sampling time, i.e., the reaction time, second; RT represents reaction temperature, and CE, concentration of enzyme HRP, mol/L.