Table 2 - Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts

	Electronic Journal of Biotechnology ISSN: 0717-3458
	© 2004 by Universidad Católica de Valparaíso -- Chile
	Vol. 7 No. 1, Issue of April 15, 2004

Table 2. Comparison of the fold-increase of total RNA and MnSOD and PLD gene transcripts in wheat lines following exposure to cold temperature for seven days

	Fold-increase measurements based on gene encoding
	MnSOD		PLD
	In Plant Line^a
	442	443	442	443
Total RNA per unit mass of tissue (1)	2.7 ± 1.4	2.3 ± 1.2	2.3 ± 0.22	2.3 ± 0.22
Total RNA per unit mass of tissue, corrected for water content (2)	2.1 ± 1.1	2.1 ± 0.86	1.8 ± 0.18	1.9 ± 0.17
Gene transcript per unit mass of RNA (3)	2.5 ± 0.95	3.5 ± 0.27	2.3 ± 0.26	2.7 ± 0.39
Gene transcript per unit mass of tissue^b	6.8 ± 4.4	8.0 ± 5.2	5.3 ± 2.1	6.2 ± 2.4
Gene transcript per unit mass of tissue, corrected for water content^c	5.3 ± 3.4	7.4 ± 2.9	4.1 ± 1.8	5.1 ± 1.8

^aLines are near-isogenic for winter (442) vs. spring (443) growth habit.
^bGene transcript per unit mass of tissue = Total RNA change (1) x Gene transcript per unit mass of RNA (3).
^cCorrected gene transcript per unit mass of tissue = Corrected total RNA change (2) x Gene transcript per unit mass of RNA (3).

Fold-increase measurements based on gene encoding

MnSOD

PLD

Total RNA per unit mass of tissue (1)

Total RNA per unit mass of tissue, corrected for water content (2)

Gene transcript per unit mass of RNA (3)

Gene transcript per unit mass of tissueb

Gene transcript per unit mass of tissue, corrected for water contentc

Gene transcript per unit mass of tissue^b

Gene transcript per unit mass of tissue, corrected for water content^c