Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2004 by Universidad Católica de Valparaíso -- Chile
Vol. 7 No. 3, Issue of December 15, 2004 
 

Table 5. Thermodynamic parameters estimated by Arrhenius approach for batch formation and deactivation of CBH by cultures of Cellulomonas flavigena. Thermodynamic parameters were determined using following equation:

Ln (qp /T) = Ln(kB/h)+ ∆S*/R-∆H*/R.1/T ; where qp, T, kB , h, ∆S*, ∆H* and R are specific productivity, absolute temperature, Boltzmann constant, Planck's constant, entropy of activation, enthalpy of activation and gas constant respectively. The values of kB , h, and R are 1.38x10-23 J.K-1, 6.63 x 10-34 J.s and 8.314 J/.K-1.mol-1 respectively.

∆H* was calculated as slope and Ln(kB/h)+ ∆S*/R as intercept on Y-axis using Figure 2b.


Enzyme formation

Thermal inactivation

Activation enthalpy
(kJ/.mol)

86.9 3b

92.6 8a

Activation entropy
(J/mol/ K)

90.5 5a

(-) 498.5 25b

 

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