Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No.2, Issue of August 15, 2005 
 

Table 1. Purification of chitinase by ammonium sulphate, DEAE-Sephadex column chromatography and Sephadex G-200 gel filtration.

Purification Step

Total Activity
(U)

Total protein
(mg)

Specific activity
(U/mg)

Purification fold

Recovery
(%)

Cell free supernatant

45100

255

176.4

-

100

Dialysed (ammonium sulphate precipitation 30-75%)

31950

57

560.5

3.18

71.0

DEAE Sephadex

22500

5.5

4090.9

23.2

47.1

Gel filtration
(Sephadex G-200)

14010

1.8

7783.3

44.12

31.1

 

Supported by UNESCO / MIRCEN network