Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 3, Special Issue, 2006
 

Table 1.Formulation of culture media.

 

Basal Medium

Auxin/
Giberellin

Citokynine

Carbon source

Other additives

Callus Induction Medium (CIM)

MS/2

NAA 3 mg/L

GA3 5 mg/L

BAP 1 mg/L

3% sucrose

 

Embryogenesis Induction Medium MIE

MS full strength

 

BAP 10 mg/L

6% glucose

Adenina 40 mg/L

Putrescine 161.1 mg/L

Embryos Germination Medium (EGM)

MS full strength

GA3 0.01mg/L

BAP 0.2 mg/l

3% sucrose

Putrescine 161.1 mg/L

Secondary Embryogenesis Medium (SEM)

MS full strength

 

BAP 1 mg/L

2% sucrose

Casein hydrolyzed 200 mg/L

Putrescine 161.1 mg/L Adenine 40 mg/L


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