SciELO - Scientific Electronic Library Online

 
vol.127 número11Aseguramiento de la calidad profesional.: Un nuevo marco ético para el ejercicio de la medicinaMedicina basada en evidencias: ¿Una estrategia que acerca o aleja de la Medicina Interna? índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Revista médica de Chile

versão impressa ISSN 0034-9887

Resumo

CASTILLO N, Juan L et al. Factors that affect DNA content analysis by flow cytometry. Rev. méd. Chile [online]. 1999, vol.127, n.11, pp.1385-1397. ISSN 0034-9887.  http://dx.doi.org/10.4067/S0034-98871999001100014.

DNA ploidy and cell cycle analysis by flow cytometry is used to obtain additional information about the diagnosis and prognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation methods (mechanical, enzymatic or other), use of different dyes, lasers, analysis software with different mathematical models and interpretation of results. A discussion about the different aspects that affect the study of DNA ploidy and cell cycle and a consensus in publications is mandatory. A strict control of analysis processes and data interpretation is also necessary.

Palavras-chave : Cell Cycle; Cytological Techniques; DNA; Ploidies.

        · texto em Espanhol

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons