SciELO - Scientific Electronic Library Online

 
vol.130 número4Cambios epidemiológicos en las enfermedades infecciosas en Chile durante la década 1990-2000: 1990-2000Reducción de la mortalidad por infarto del miocardio en hospitales chilenos índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Revista médica de Chile

versión impresa ISSN 0034-9887

Resumen

SANCHEZ, Gittith et al. Immunophenotyping by flow cytometry of peripheral blood lymphocytes from Chilean chronic chagasic patients. Rev. méd. Chile [online]. 2002, vol.130, n.4, pp.363-367. ISSN 0034-9887.  http://dx.doi.org/10.4067/S0034-98872002000400002.

Background: Cellular immune mechanisms of the resistance to infection by T cruzi as well as the pathogenesis of Chagas disease are still controversial. Aim: To quantify and analyse the peripheral blood immune cells from chagasic and non chagasic patients by flow cytometry. Patients and methods: Peripheral blood samples were taken from 21 individuals seropositive for Chagas disease, under no specific treatment. Control samples from 21 healthy blood donors were also obtained. To quantify immune cells populations by flow cytometry, antibodies against CD3, CD4, CD8, CD16/56, CD45/14, CD19 and HLA-DR markers were used. Results: The percentage of CD8+ cells was low and the CD4+/CD8+ ratio was high in chagasic patients, compared to their non infected counterparts. No statistically significant differences in the number of CD4+, NK, B, CD4+HLADR+ and CD8+HLADR+ cells, were observed within the two groups. Conclusions: These results show that Chilean chronic chagasic patients have lower percentage of CD8+ cells and higher CD4+/CD8+ ratio than non infected individuals (Rev Méd Chile 2002; 130: 363-367

Palabras clave : CD4-positive T-lymphocytes; CD4-CD8 ratio; CD8-positive T-lymphocytes; Chagas disease; Flow cytometr.

        · texto en Español

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons