SciELO - Scientific Electronic Library Online

 
vol.130 número6Perfil del hipertenso adulto mayor tratadoSíndrome de Williams: estudio clínico, citogenético, neurofisiológico y neuroanatómico índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Revista médica de Chile

versão impressa ISSN 0034-9887

Resumo

ARTIGAS A, Carmen Gloria et al. Detection of BCR-ABL gene sequences using polymerase chain reaction reverse transcriptase in patients with leukemia. Rev. méd. Chile [online]. 2002, vol.130, n.6, pp.623-630. ISSN 0034-9887.  http://dx.doi.org/10.4067/S0034-98872002000600004.

Background: The BCR-ABL fusion gene is the molecular expression of the Philadelphia chromosome. This cytogenetic aberration is the most frequent alteration found in leukemias, which is produced by the translocation t(9;22). Two different fusion proteins are produced depending on the break point (210 kD and 190 kD). The detection of this gene has both diagnostic and prognostic importance, associated with poor prognosis in acute lymphoblastic leukemia (ALL). Aim: To detect BCR-ABL gene sequences in patients with leukemia from the IX Region of Chile. Material and methods: We studied 58 patients: 5 chronic myeloid leukemia (CML), 35 ALL, 15 acute myeloid leukemia (AML) and 3 biphenotypic leukemia. The gene sequences were detected using reverse transcriptase polymerase chain reaction (RT-PCR). Results: BRC-ABL gene sequences were positive in all patients with CML, 2 of 35 ALL (one child and one adult). The remaining patients were negative. We found p210 and p190 co-expression in 2 CML and 1 ALL. Conclusions: Our results are in agreement with other reports. The detection of these and other genetic alterations will allow us to have invaluable diagnostic and prognostic information from our patients with leukemia (Rev Méd Chile 2002; 130: 623-30)

Palavras-chave : Leukemia; Oncogene proteins; fusion; Philadelphia chromosome; Reverse transcriptase polymerase chain reaction.

        · texto em Espanhol

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons