SciELO - Scientific Electronic Library Online

 
vol.130 número12Carcinoma subseroso de la vesícula biliar: expresión del complejo caderina-cateninaAlta prevalencia de osteoporosis en mujeres mapuches postmenopáusicas asintomáticas índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Revista médica de Chile

versión impresa ISSN 0034-9887

Resumen

MERCADO P, Rubén et al. Immunodiagnosis of Strongyloides stercoralis infections in Chile using an Elisa test. Rev. méd. Chile [online]. 2002, vol.130, n.12, pp.1358-1364. ISSN 0034-9887.  http://dx.doi.org/10.4067/S0034-98872002001200005.

Background: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated disease may appear, sometimes fatal. In Chile, there is little epidemiological information about S stercoralis infections and appropriate diagnostic techniques are usually not used. Aim: To evaluate the yield of an ELISA test for the diagnosis of strongyloidiasis in Chilean patients. Material and methods: Ten serum samples from patients with S stercoralis infections confirmed by a positive stool examination, 66 samples from individuals with other infections by tissue helminthes (24 toxocariasis, 15 trichinellosis, 11 hydatidosis, 12 fascioliasis and 4 cysticercosis), 13 samples from subjects with autoimmune diseases and 49 samples from apparently healthy individuals with a normal eosinophil count, were studied. ELISA antigen was prepared using a filariform larval extract obtained from a murine species of Strongyloides, maintained in laboratory animals. Results: Using 0.33 optical density units as a cut off value, 9 of 10 sera of S stercoralis infected individuals, had a positive ELISA test. No cross reactions were observed with sera of patients with other helminthic infections, autoimmune diseases or in healthy individuals. Thus, specificity, positive and negative predictive values were 100%. Conclusions: The results obtained are similar with those found by other investigators. ELISA test for strongyloidiasis is a useful tool for the diagnosis of clinical cases and for seroepidemiological studies of this nematode infection in Chile (Rev Méd Chile 2002; 130: 1358-64)

Palabras clave : Enzyme linked immunosorbent assay; Nematode infections; Strongyloides stercoralis.

        · texto en Español

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons