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Revista médica de Chile

versão impressa ISSN 0034-9887

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ROA, Iván; SANCHEZ, Tamara; MAJLIS, Alejandro  e  SCHALPER, Kurt. KRAS gene mutation in colorectal cancer. Rev. méd. Chile [online]. 2013, vol.141, n.9, pp.1166-1172. ISSN 0034-9887.  http://dx.doi.org/10.4067/S0034-98872013000900009.

Background: KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. Aim: To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and Methods: KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing. Results: Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location. Conclusions: In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation.

Palavras-chave : Colonic neoplasms; Rectal neoplasms; Sarcospan (Kras oncogene-associated gene) protein, human.

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