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Archivos de medicina veterinaria

versión impresa ISSN 0301-732X


SABINI, L.I. et al. Referential diagnostic techniques to analyze virulence markers of pseudorabies virus RC/79 strain\: preliminary assays. Arch. med. vet. [online]. 1997, vol.29, n.2, pp.250-260. ISSN 0301-732X.

The indirect immunoperoxidase and immunofluorescence methods were standardized for the detection of Aujeszky's disease virus in Vero cell cultures, using as a model the RC/79 strain virus." There was a strong correlation in the results obtained by both methods. The results proved to be highly specific for the identification of the virus and allowed a precise localization of the viral antigen in infected cells. The presence of syncytia with a strong reaction in the nuclear region 16 hours p.i. indicated that RC/79 strain is strongly virulent. This conclusion was supported by the results obtained from experimental rabbit infection. The animals were inoculated with different doses of the virus (102-105 PFU). In all the cases the mortality rate was 100%, even when animals received minor doses of the virus, indicating no dose response. The mean death time was 62 and 120 hours p.i. for the different doses used. Between 6 and 24 hours prior to death, all rabbits presented an exudative lesion in the inoculation area and extensive pruritus. The methods employed were useful as virulence markers of the RC/79 strain virus and on the other hand, they constitute essential tools in the diagnosis of Aujeszky's disease virus

Palabras clave : Aujeszky's virus; RC/79 strain; virulence markers; immunohistochemistry.

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