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Archivos de medicina veterinaria

versión impresa ISSN 0301-732X

Resumen

ZAMORA, J.; REINHARDT, G.; POLETTE, M.  y  ORELLANA, C. M.. Escherichia coli isolated from feces of diarrheic piglets. Patterns of adherence to HEp-2 cells. Arch. med. vet. [online]. 2000, vol.32, n.2, pp.245-251. ISSN 0301-732X.  http://dx.doi.org/10.4067/S0301-732X2000000200013.

Diarrheic feces from 150 piglets younger than 40 days old, were examined in order to isolate E. coli strains and test their adherence capacity to HEp-2 cells. For this purpose, samples were seeded on eosin methylen blue agar. Isolated E. coli strains were cultured in tryptone broth with 1% D-mannose and then inoculated to HEp-2 monolayer cells.. Strains with adherence capacity were obtained from 20 piglets. In 11 piglets (7.3%) the isolated strains showed localised adherence; in 5 piglets (3.3, they had an aggregative pattern of adherence and in 4 piglets (2.7%), they showed a pattern of diffuse adherence

Palabras clave : E. coli; LAEC; DAEC; EAggEC; diarrhoea; piglets.

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