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Archivos de medicina veterinaria

versión impresa ISSN 0301-732X

Resumen

RAMIREZ-MERLANO, JA; MEDINA-ROBLES, VM  y  CRUZ-CASALLAS, PE. Seminal Cryopreservation of bagre rayado Pseudoplatystoma metaense (Teleostei, Pimelodidae), under different protocols of freezing. Arch. med. vet. [online]. 2011, vol.43, n.2, pp.135-144. ISSN 0301-732X.  http://dx.doi.org/10.4067/S0301-732X2011000200006.

The aim was to determine the effect of different extenders and freezing rates on bagre rayado seminal quality. Sexually mature males (1.5 ± 0.2 kg and 56.6 ± 2.9 cm) and females (2.8 ± 0.7 kg and 71.3 ± 6.1 cm) were used. Ovulation and spermiation were induced with Carp Pituitary Extract. The semen was diluted (1:6, semen: diluent) with a glucose-based solution (5.5%) and egg yolk (YH, 12%) or whole milk powder (LE, 5%). As cryoprotectants dimethyl sulfoxide (DMSO, 10%), methanol (MET, 12%) or dimethyl acetamide (DMA, 10%) were used. The diluted semen was packaged in 0.5 mL straws and frozen in two freezing curves (C1= 2.4 °C·min-1 from 28 to -4 °C, followed by 10.0 °C·min-1 from -4 to -80 °C and C2= 8.4 °C·min-1 of 4 to -80 °C) and then immersed in liquid nitrogen (-196 °C). The straws were thawed in a water bath at 35 °C by 60 sec and immediately assessed motility. The fertility was assessed through semination of 2 grams of oocytes with 100 or 400 µL of fresh or frozen semen, respectively, and determined at 6:00 h postfertilization. The results showed higher rates of fertility with fresh semen, fertilized oocytes were observed in all treatments, with greater percentage (greater than 60%) from the semen under the C2 (greater than 60%) and 10 or 12% of DMSO or MET, respectively and 5.5% glucose and 5% of LE. These results allow to establish an acceptable freezing protocol for the artificial reproduction of bagre rayado.

Palabras clave : cryopreservation; spermatozoa; Pseudoplatystoma metaense; sperm.

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