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Archivos de medicina veterinaria

versión impresa ISSN 0301-732X

Resumen

GONZALEZ, JC; LOPEZ, C  y  CARMONA, JU. Evaluation of a manual method for producing pure-platelet rich plasma (P-PRP) in rabbits: hematological study. Arch. med. vet. [online]. 2013, vol.45, n.3, pp.267-272. ISSN 0301-732X.  http://dx.doi.org/10.4067/S0301-732X2013000300006.

The objective of this study was to evaluate a manual method to produce two kinds of pure-platelet rich plasma (P-PRP-A and P-PRP-B) in rabbits. Both P-PRP were obtained by drawing blood from the jugular vein into tubes containing sodium citrate as anticoagulant and, centrifuged at 259 g for 6 minutes. The number of concentrated platelets in the P-PRP-A was 716.14 X 103/μL and 630 X 103/μL for P-PRP-B. Cell counts were significantly different (P < 0.05) between whole blood and PRP fractions A and B, for RBC counts (106/μL), WBC (103/μL), PLT (103/μL) and PDW (%) but not for MPV (fL). Gender differences were found in P-PRP-A for RBC (106/μL), which was higher in males, and the MPV (fL), which was higher in females. In fraction P-PRP-B a difference was found for MPV (fL), which was higher in females. The PDW (%) was higher in males compared with females. The platelet collection efficiency was 20.76% for P-PRP-A and 18.27% for P-PRP-B. The platelet concentration considering whole blood was 186.92% for P-PRP-A and 164.44% for P-PRP-B. The protocol described in this study represents a simple method to obtain P-PRP in rabbits for experimental purposes and even for therapeutic use.

Palabras clave : rabbit; platelet concentrate; tube method; cellular study; regenerative therapy.

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