SciELO - Scientific Electronic Library Online

 
vol.46 número2Bacteriófagos líticos en Medicina Veterinaria: ¿una opción terapéutica frente a patógenos bacterianos?Efecto de la suplementación de progesterona y enfriamiento artificial post-inseminación sobre la eficiencia reproductiva de vaquillas Holstein durante el verano índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Archivos de medicina veterinaria

versión impresa ISSN 0301-732X

Resumen

MUNOZ-BANALES, LA et al. Two new miRNAs identified in bovine ovary. Arch. med. vet. [online]. 2014, vol.46, n.2, pp.181-188. ISSN 0301-732X.  http://dx.doi.org/10.4067/S0301-732X2014000200003.

The microRNAs (miRNAs) that participate in regulating oocyte growth and oocyte-embryo transition must be identified and characterised to understand maturation, fertilization and embryo development mechanisms in bovine reproduction. With the purpose of identifying new miRNAs that may play a role in oocyte maturation and early embryogenesis, a miRNAs genome library from bovine ovarian was developed. After analysing concatamers from 40 clones, two new miRNAs were identified and labeled bta-mir-8548 and bta-mir-8549. These miRNAs showed a stable stem-loop structure in accordance with established miRBase parameters. bta-mir-8548 was found at chromosome 8 in an intronic region between exons 1 and 2 of the MRSA gene (methionine sulfoxide reductase) suggesting that it may play a role in regulating oxidative stress during oocyte maturation. In the same way, bta-mir-8549 was found at chromosome 7 in the intergenic region between genes tRNA-Cys and GBPg5. The mature form of bta-mir-8549 showed the conserved sequence of the bta-let-7 family, which is associated with oocyte maturation. With the purpose of locate the correct place of bta-mir-8549 into the family of miRNA bta-let-7 a phylogenetic study was conducted. The cladogram generated from genetic distances between the various bta-let-7 miRNAs showed that bta-mir-8549 was located monophyletically with bta-lec-7c, while the rest of the bta-let-7 miRNAs were located in different clades. It was concluded that bta-mir-8548 and bta-mir-8549 are new bovine ovarian miRNAs that may act on the mechanisms regulating oocyte maturation and embryonic development.

Palabras clave : microRNAs; oocyte; embryonic development.

        · resumen en Español     · texto en Español     · Español ( pdf )