Resumen

BARTICEVIC R., Margarita et al. Phenotypic characterization of microsatellite-fingerprinted segregants, focused on seedlessness and giberelic acid response on berry size of grapes. Agric. Téc. [online]. 2004, vol.64, n.1, pp.3-16. ISSN 0365-2807.  http://dx.doi.org/10.4067/S0365-28072004000100001.

In this article the characterization of a number of table grape (Vitis vinifera L.) crossings is reported on the development of seedlessness, berry size, and response to giberelic acid (GA3) treatment on berry growth. To do so, segregants of known identity have been used and their affiliation has been verified based on a group of five microsatellite markers selected because of their high heterozygocity evaluated on the progenitors. These markers detected between 18 and 21% of plants coming from mother self-pollination events, as well as 3% of genotypes non-related to either progenitor. The crosses studied were Ruby Seedless x Sultanina, Ruby Seedless x Perlette and their corresponding reciprocals (76 and 56 seedlings in production, respectively), as well as Black Seedless x Flame Seedless (23 seedlings in production). Forty percent of seeded descendants were identified among the different crosses, a value similar to that reported in other breeding programs that have used seedless progenitors. For all of the constitutively expressed traits evaluated (seed weight and rudiment total weight and berry size) continuous segregation and positive transgression with respect to the progenitors were detected, particularly in seed size. The size of seeds or rudiments and berries exhibited an appropriate reproducibility between seasons, contrasting with the response to GA3, which was unstable during the three evaluation seasons. In spite of that, seedlings were found that regularly had a very interesting combination, which is having negligible presence of seed rudiments and large berry size (15 to 19 mm).

Palabras clave : Vitis vinifera L.; genetic improvement of table grapes; affiliation; microsatellites; seedlessness; giberelic acid; berry size.