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Agricultura Técnica

Print version ISSN 0365-2807

Abstract

RIOS L, Darcy et al. Rooting Rate Variation Related to Subculture Number and Diameter of Chestnut Castanea sativa Mill. Microshoots. Agric. Téc. [online]. 2005, vol.65, n.3, pp.258-264. ISSN 0365-2807.  http://dx.doi.org/10.4067/S0365-28072005000300003.

This study had the objective of determining  the subculture number and the shoot basal diameter that produces the best  rooting rate ex vitro of chestnut, Castanea sativa Mill., microshoots obtained via in vitro culturing.  The plant material corresponded to  microcuttings obtained from embryo cultures with between 7 and 12 subcultures in the proliferative  stage, for which Driver and Kuniyuki Walnut (DKW)  medium was used with the macronutrients reduced to a half and  supplemented with 1 mg L-1 6-benzylaminopurine (BAP) and 0.1 mg L-1 indole-3-butiric acid (IBA). The quick induction rooting method was used, submerging the base of the microcuttings in a solution of 0.5 mg mL-1 of IBA for 15 min. For the rooting stage, pine bark:perlite (4:1, v/v) substrate was used, carrying out the evaluation of results at 20 days after culturing. The evaluated variables were survival rate (%), rooting rate (%), root number, root length (mm), callus presence and visual aspect of the rooting system. The results showed a decrease of rooting capacity as the subculture number increases. The factor microcutting diameter did not present significant differences  with respect to the evaluated variables.

Keywords : chestnut; micropropagation; in vitro culture.

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