Resumo

MERINO, M.; NUNEZ-VERGARA, L.J.  e  SQUELLA, J.A.. Cytochrome C reductase immobilized on carbon paste electrode and its electrocatalytic effect on the reduction of cytochrome C. Bol. Soc. Chil. Quím. [online]. 2000, vol.45, n.3, pp.433-439. ISSN 0366-1644.  http://dx.doi.org/10.4067/S0366-16442000000300014.

ABSTRACT Carbon paste electrodes were modified with cytochrome c reductase enzyme. The behavior of the electrodes was characterized by cyclic voltammetry. The cytochrome c reduction was investigated both on the enzyme modified carbon paste electrode and in a homogeneous system. In the first case, a significant electrocatalytic effect on the reduction of cytochrome c was found. The effect is supported by an increase of 4.3 fold of the peak current and a shift of the cathodic peak potential toward less cathodic values. Also, apparent Michaelis constant values were calculated. Results from this work show that carbon paste electrode modified by the cytochrome c reductase represents a new interface that will permit the study of electron transfer reactions involved in biodegradation processes.

Palavras-chave : Cytocrome c reductase; electrocatalysis; modified carbon paste electrode.