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vol.47 número1OPTIMIZACIÓN DEL EXPONENTE IMPLÍCITO EL EL ALGORITMO CORRESPONDIENTE AL INDICE DE CONECTIVIDAD DE VALENCIA TRIDIMENSIONAL COREGIDO lambda (q).APLICACIÓN A HIDROCARBUROS SATURADOSEFFECTS OF SOLAR UV-B RADIATION IN THE ACCUMULATION OF RHIZOCARPIC ACID IN A LICHEN SPECIES FROM ALPINE ZONES OF CHILE índice de autoresíndice de assuntospesquisa de artigos
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Boletín de la Sociedad Chilena de Química

versão impressa ISSN 0366-1644

Resumo

FIGUEROA, Carolina E. et al. ANÁLISIS DE LA FRACCCIÓN LIPÍDICA DE LA YEMA DE HUEVOS POR AMD-HPTLC. Bol. Soc. Chil. Quím. [online]. 2002, vol.47, n.1, pp.61-66. ISSN 0366-1644.  http://dx.doi.org/10.4067/S0366-16442002000100011.

An Instrumental Planar Chromatographic Method (HPTLC) with Automatic Multiple Development Chamber (AMD) for the cholesterol analysis in egg yolk obtained from hens fed with diet to reduce the cholesterol content, was set up and validated. The gradient to achieve the simultaneous separation, identification and quantification of cholesterol, phosphatidylcholine and triolein was established. Recovery was evaluated through a standard addition procedure with a yielding of 99,4%. Repeatability and Intermediate Repeatability resulted adequate on the ground of Media Difference analysis at a confidence limit of 95%.

Palavras-chave : Cholesterol; phosphatidylcholine; triolein; HPTLC-AMD; densitometry.

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