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Parasitología al día

versão impressa ISSN 0716-0720

Resumo

GORMAN, TEXIA; LOPEZ, CLAUDIA; FREDES, FERNANDO  e  ALCAINO, HECTOR. IMMUNOLOGICAL MONITORING OF CHEMOTHERAPEUTIC SUCCESS IN OVINE FASCIOLOSIS USING A SEMIPURIFIED ANTIGEN OF < 30KDa. Parasitol. día [online]. 2000, vol.24, n.1-2, pp.27-34. ISSN 0716-0720.  http://dx.doi.org/10.4067/S0716-07202000000100004.

The chemotherapeutic success of a semipurified Fasciola hepatica antigenic fraction of < 30kDa was evaluated in naturally infected sheep using ELISA and a Western blot (WB) assay. Sheep without fasciolosis were sent and kept in pastures where the infection is highly endemic. Serum samples from 15 sheep were monthly evaluated from day 0 to 12 months of infection by sedimentation coprological exams, ELISA and WB. Six months post infection (p.i.), six animals were treated with triclabendazole. Serum samples from sheep free of fasciolosis, sera from infected sheep and from sheep with other parasitic diseases were included as controls. Results obtained, indicated that the treatment effect became obvious by a reduction of antibody levels and a seroconversion to negative of 5 sheep after six months post treatment, however differences in the proportions of positive ELISA results among treated and control animals, became significant earlier, at 4 months post treatment (p < 0.05). The WB assays indicated that at 2 months after treatment, half of the treated sheep did not recognized bands < 30 kDa and a 14 kDa band (both being considered through previous studies as highly sensitive and specific in the diagnosis of fasciolosis). Differences in the proportion of positives in treated and control groups were already statistically significant at 2 months after treatment (p < 0.05). At 5 months after treatment, there was no recognition of both bands in any of the treated group. It was demonstrated through coprological exams and the post mortem examination of the animals, the absolute absence of infection in treated sheep and the presence of F. hepatica infection in control groups. It is therefore concluded that the semipurified fraction < 30 kDa contains polypeptides which might be veryuseful not only from a diagnostic point of view, but also as indicators in predicting chemotherapeutic success in animals treated against fasciolosis.

Palavras-chave : Fasciola hepatica immunodiagnosis; fasciolosis chemothera-peutic success; Western blot; ELISA; sheep fasciolosis.

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