SciELO - Scientific Electronic Library Online

 
vol.24 número3-4Tripomastigotes de sangre y de cultivo celular de Trypanosoma cruzi Y.: II.- Patologíade la enfermedad de Chagas en ratones Balb/c índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Parasitología al día

versão impressa ISSN 0716-0720

Resumo

BORNAY-LLINARES, FERNANDO J. et al. MANTAINED IN CULTURE AND CHARACTERIZATION OF A MICROSPORIDIAN (Encephalitozoon hellem) ISOLATED FROM AIDS PATIENT WITH NEUMONIA. Parasitol. día [online]. 2000, vol.24, n.3-4, pp.69-70. ISSN 0716-0720.  http://dx.doi.org/10.4067/S0716-07202000000300001.

We report the identification to the species level of a microsporidian isolated from the bronchoalveolar lavage of an AIDS patient in Spain. Characterization of the isolate was performed by: 1) morphological methods using optical and electron microscopy; 2) immunological methods such as immunofluorescence assay (IIF) and immunoblot (WB) using specific antiserum and, 3) polymerase chain reaction (PCR) using species-specific primers designed on the coding region of the small subunit of the ribosomal RNA gene. Ultrastructural features allowed the identification of the isolate as belonging to the Encephalitozoon genus. Species identification as Encephalitozoon hellem was achieved by IIF and WB using polyc lonal rabbit serum anti-E. hellem (CDC:0291:V213), as well as, by PCR using EHELF/EHELR species-specific primers. The isolate has been named EHVS-96 and has been maintained for 3 years in Vero-E6 monolayers. This is the first isolation in culture and characterization of E. hellem in Spain.

Palavras-chave : Human microsporidiosis; AIDS; Encephalitozoon hellem; Culture.

        · resumo em Espanhol     · texto em Espanhol

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons