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Revista chilena de infectología

versión impresa ISSN 0716-1018

Resumen

GUEVARA, Armando; DE WAARD, Jacobus  y  ARAQUE, María. blaVIM-2 gene detection in metallo-ß-lactamase-producing Pseudomonas aeruginosa strains isolated in an Intensive Care Unit in Ciudad Bolívar, Venezuela. Rev. chil. infectol. [online]. 2009, vol.26, n.4, pp.336-341. ISSN 0716-1018.  http://dx.doi.org/10.4067/S0716-10182009000500005.

Ten Pseudomonas aeruginosa strains with resistance to broad-spectrum cephalosporin and carbapenems were studied to determine the presence of genes that mediate the productionof metallo-p-lactamases. These strains were isolated from patients with nosocomial infection at the Intensive Care Unit of the Complejo Hospitalario "Ruiz y Paéz" of Ciudad Bolívar, Bolívar State, Venezuela, from 2003 to 2006. In all isolates a metallo-enzyme activity was detected by using the double disk synergism test. PCR amplification of genes encoding the families IMP, VIM and SPM metallo-ß-lactamases showed the presence of a blaVIM gene in all strains studied. DNA sequencing revealed that all isolates showed the presence of blaVIM-2These results suggest that it is necessary to keep these strains under epidemiologic surveillance, establish laboratory strategies for opportune detection and the imple-mentation of new policies to ensure the appropriate use of antibiotics in this institution.

Palabras clave : Pseudomonas aeruginosa; metallo-ß-lactamases; blaVIM-2 gene; nosocomial infections.

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