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Revista chilena de infectología

versión impresa ISSN 0716-1018

Resumen

SELMAN B, Carolina et al. Assessment of the Amplicor PCR® for the detection of Mycobacterium tuberculosis in smear negative respiratory and non respiratory specimens. Rev. chil. infectol. [online]. 2009, vol.26, n.6, pp.495-498. ISSN 0716-1018.  http://dx.doi.org/10.4067/S0716-10182009000700001.

Background: Commercial polymerase chain reaction (PCR) kits are widely accepted for analysis of smear positive respiratory specimens, but the sensitivity is variable for smear negative ones. Objective: To assess the PCR method usefulness in smear negative respiratory and non respiratory specimens. Methods: We compared the PCR results (AMPLICOR MTB test™, Roche) of 235 specimens subjected to culture in Loewenstein-Jensen agar (as the gold standard). Results: 181 (76%) were respiratory and 54 (24%) extra-respiratory specimens. The sensitivity was 88%) and 50%>, respectively, specificity and PPV was 100%> in both cases. NPV was 99.4%> in respiratory specimens and 96.1% in non-respiratory specimens. Conclusions: The good performance of this PCR in smear negative respiratory specimens allows the clinician to take decisions based on the result of this exam. In extra-respiratory specimens the contribution is important only when the PCR result is positive.

Palabras clave : Polymerase chain reaction; molecular diagnostic testing; Mycobacterium tuberculosis; tuberculosis.

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