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Revista chilena de infectología

versão impressa ISSN 0716-1018

Resumo

BUSTAMANTE, Verónica; MEZA, Paulina; ROMAN, Juan C  e  GARCIA, Patricia. Evaluation of an automated streaking system of urine samples for urine cultures. Rev. chil. infectol. [online]. 2014, vol.31, n.6, pp.670-675. ISSN 0716-1018.  http://dx.doi.org/10.4067/S0716-10182014000600005.

Introduction: Automated systems have simplified laboratory workflow, improved standardization, traceability and diminished human errors and workload. Although microbiology laboratories have little automation, in recent years new tools for automating pre analytical steps have appeared. Objectives: To assess the performance of an automated streaking machine for urine cultures and its agreement with the conventional manual plating method for semi quantitative colony counts. Materials and Methods: 495 urine samples for urinary culture were inoculated in CPS® agar using our standard protocol and the PREVI™ Isola. Rates of positivity, negativity, polymicrobial growth, bacterial species, colony counts and re-isolation requirements were compared. Results: Agreement was achieved in 98.97% of the positive/negative results, in 99.39% of the polymicrobial growth, 99.76% of bacterial species isolated and in 98.56 % of colony counts. The need for re-isolation of colonies decreased from 12.1% to 1.1% using the automated system. Discussion: PREVI™ Isola's performance was as expected, time saving and improving bacterial isolation. It represents a helpful tool for laboratory automation.

Palavras-chave : Urine culture; streaking; automatization; Previ Isola; microbiology.

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