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Revista chilena de infectología

versão impressa ISSN 0716-1018

Resumo

ULLOA, María Teresa et al. Chilean strains of clinical origin of non-O1, non-O139 Vibrio cholerae carry the genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF from secretion system T3SS2 present in an island of pathogenicity. Rev. chil. infectol. [online]. 2019, vol.36, n.3, pp.312-317. ISSN 0716-1018.  http://dx.doi.org/10.4067/S0716-10182019000300312.

Backgound:

The virulence factors of the Vibrio cholerae non-O1, non-O139 strains are not clearly known. The strain of septicemic origin NN1 Vibrio cholerae non-O1, non-O139 was sequenced previously by the Illumina platform. A fragment of the pathogenicity island VPaI-7 of V. parahaemolyticus was detected in its genome.

Aim:

To detect the virulence genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF in Chilean strains of V. cholerae non-O1, non-O139.

Methods:

A total of 9 Chilean strains of clinical origin of Vibrio cholerae non-O1, non-O139 isolated between 2006-2012 were analyzed by conventional PCR assays for type III secretion genes encoded on that island: vcsN2, vcsC2, vcsV2, vspD, toxR2 and vopF. Additionally, the presence of the virulence genes hylA and rtxA was determined. In addition, REP-PCR and ERIC-PCR assays were performed.

Results:

most (6/9) Chilean V. cholerae non-O1, non-O139 strains contain the type III secretion genes vcsN2, vcsC2, vcsV2, vspD, toxR2 and vopF, encoded in an island of pathogenicity. In addition, all (9/9) the strains contain the virulence genes hylA and rtxA.

Conclusion:

These results strongly suggest the possibility that those strains possess an important virulence potential in humans.

Palavras-chave : Chile; gastroenteritis; Vibrio cholerae non-O1; genomic islands; virulence factors; T3SS2.

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