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Revista chilena de infectología

versión impresa ISSN 0716-1018


FARFAN, Mauricio J. et al. Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing. Rev. chil. infectol. [online]. 2020, vol.37, n.3, pp.276-280. ISSN 0716-1018.

The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, we found that pooling of 5 samples showed that CT variations were in the range of 1.0-4,5 units, with less likelihood of a false negative result. Results of the sample without nucleic acid ex-traction, was unsatisfactory, with a significant increase in CT values, and thus for risk of a false negative result. In conclusion, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.

Palabras clave : coronavirus; SARS-CoV-2; COVID-19; diagnosis; pool testing.

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