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Biological Research

Print version ISSN 0716-9760

Abstract

ZAGO, M. PAOLA; VERSTRAETEN, SANDRA V.  and  OTEIZA, PATRICIA I.. Zinc in the prevention of Fe2initiated lipid and protein oxidation. Biol. Res. [online]. 2000, vol.33, n.2, pp.143-150. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602000000200014.

In the present study we characterized the capacity of zinc to protect lipids and proteins from Fe2+-initiated oxidative damage. The effects of zinc on lipid oxidation were investigated in liposomes composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS) at a molar relationship of 60:40 (PC:PS, 60:40). Lipid oxidation was evaluated as the oxidation of cis-parinaric acid or as the formation of 2-thiobarbituric acid-reactive substances (TBARS). Zinc protected liposomes from Fe2+ (2.5-50 mM)-supported lipid oxidation. However, zinc (50 mM) did not prevent the oxidative inactivation of glutamine synthelase and glucose 6-phosphate dehydrogenase when rat brain superntants were oxidized in the presence of 5 mM Fe2+ and 0.5 mM H2O2 .We also studied the interactions of zinc with epicatechin in the prevention of liid oxidation in liposomes. The simulaneous addition of 0.5 mM epicatechin (EC) and 50 mM zinc or EC separately. Zinc (50 mM) also protecte liposomes from the stimulatory effect of aluminum on Fe2+-initiated lipid oxidation. Zinc could play an important role as an antioxidant in biological systems, replacing iron and other metals with pro-oxidant activity from binding sites and interacting with other components of the oxidant defense system.

Keywords : aluminum; catechins; free radicals; iron; lipid peroxidation; zinc.

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