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Biological Research

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LOTITO, SILVINA B  and  FRAGA, CESAR G. Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma. Biol. Res. [online]. 2000, vol.33, n.2, pp.151-157. ISSN 0716-9760.

We evaluated the interaction between ascorbic acid (AA) and (+)-catechin (CTCH) in potassium phosphate solution, pH 7.4 (PPS) and in human plasma. In both systems, the oxidation was started by adding 2,2’-azobis-(2-amidinopropane) clorhidrate (AAPH). The concentrations of AA and CTCH were determined by HPLC using electrochemical detection. In PPS, CTCH was oxidized by AAPH (50 mM), in either the absence or presence of different initial concentrations of AA (25-200 mM). In the presence of AA, CTCH depletion was delayed, an effect that was dependent upon the initial concentration of AA. When 100 mM AA was added after the oxidation had begun, CTCH depletion was arrested for 30 min. The kinetics of AA oxidation by AAPH was also characterized in PPS. AA (100 mM) was completely consumed after 60 min of reaction at 37 mC, in both the absence and presence of 100 mM CTCH. When human plasma was incubated with 50 mM AAPH in the absence of added CTCH, AA was completely consumed after 45-60 min. CTCH did not prevent AA depletion in human plasma at the concentrations tested (10, 50 100 mM). The results point out that AA is able to protect other aqueous soluble antioxidants, e.g.: CTCH

Keywords : catechins; antioxidants; free radicals; procyanidins.

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