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Biological Research

Print version ISSN 0716-9760

Abstract

GOTTE, MARTIN  and  STADTBAUMER, ANDREA. Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae. Biol. Res. [online]. 2002, vol.35, n.3-4, pp.347-357. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602002000300009.

The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable of complementing the sorting and maturation defects of the vacuolar hydrolase CPY in pep12 null mutants. No dominant negative effects of either syntaxin 6 or syntaxin 6DC overexpression on CPY sorting and maturation were observed in wild-type yeast cells. We conclude that syntaxin 6 and Pep12p do not act at the same vesicular trafficking step(s) in yeast and higher eukaryotes

Keywords : vesicular transport; SNARE; syntaxin; endosomal trafficking; vacuole protein sorting; PEP12.

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