SciELO - Scientific Electronic Library Online

 
vol.37 issue1Impact of the Training Program on Lipid Profile and Cardiac Health author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Biological Research

Print version ISSN 0716-9760

Abstract

CARRILLO, JUAN C; OJEDA, VERÓNICA A; CAMPOS-DE QUIROZ, HUGO A  and  ORTEGA, FERNANDO M. Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes. Biol. Res. [online]. 2004, vol.37, n.1, pp.45-51. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602004000100005.

A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contamination and a better development than crown meristems. The L2 medium showed better results than B5 and MS media for the cultivars and experimental lines studied. L2 medium supplemented with 0.003 mg/l of 4-amino-3,5,6-trichloropicolinic acid and 1.0 mg/l of 6-benzylaminopurine gave consistently better results and will be applied in our breeding program to propagate, maintain and eliminate viruses from elite red clover clones.

Keywords : In vitro culture; meristem culture; red clover; Trifolium pratense L.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License