SciELO - Scientific Electronic Library Online

vol.37 número3The Gonyautoxin 2/3 epimers reduces anal tone when injected in the anal sphincter of healthy adultsRole of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Biological Research

versión impresa ISSN 0716-9760


MORENO, ISABEL M; PEREIRA, PAULO; FRANCA, SUSANA  y  CAMEAN, ANA. Toxic cyanobacteria strains isolated from blooms in the Guadiana River (southwestern Spain). Biol. Res. [online]. 2004, vol.37, n.3, pp.405-417. ISSN 0716-9760.

This paper describes the occurrence of toxic cyanobacteria along the Guadiana River over its course between Mérida and Badajoz (Extremadura, Spain). Water sampling for phytoplankton quantification and toxin analysis was carried out regularly between 1999 and 2001 in six different locations, including two shallow, slow-flowing river sites, two streamed river sites and two drinking water reservoirs. The cyanobacterial community differed significantly between these locations, especially during the summer. The predominant genera were Microcystis, Oscillatoria, Aphanizomenon and Anabaena. Using an ELISA assay the total microcystin contents of natural water samples from the most eutrophic locations ranged from 0.10 _ 21.86 mg mcyst-LR equivalent·L-1 in Valdelacalzada and 0.10-11.3 µg mcyst-LR equivalent·L-1 in Vitonogales, and a seasonal variation of toxin content was observed. The amount of microcystins produced by each strain was determined by ELISA assay and the detection and identification of microcystin variants of three toxic strains of Microcystis aeruginosa was performed by high performance liquid chromatography (HPLC). The analysis of microcystins of the cultured strains revealed that toxin production was variable among different strains of M. aeruginosa isolated either from different blooms or from the same bloom.

Palabras clave : Cyanobacteria; microcystins; Guadiana; Spain.

        · texto en Inglés     · Inglés ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons