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Biological Research

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DEMURO, ANGELO  and  PARKER, IAN. Imaging single-channel calcium microdomains by total internal reflection microscopy . Biol. Res. [online]. 2004, vol.37, n.4, pp.675-679. ISSN 0716-9760.

The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes

Keywords : single-channel recording; calcium imaging; TIRFM; N-type Ca2+ channels.

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