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Biological Research

Print version ISSN 0716-9760

Abstract

URBINA, DANIELA C; SILVA, HERMAN  and  MEISEL, LEE A. The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana. Biol. Res. [online]. 2006, vol.39, n.2, pp.289-296. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602006000200011.

Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism

Keywords : Arabidopsis thaliana; Ca2+-ATPases; calcium; gravitropism; thapsigargin; root.

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