SciELO - Scientific Electronic Library Online

 
vol.39 issue2The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thalianaHypercholesterolemia and tissue-specific differential mRNA expression of type-1 5'-iodothyronine deiodinase under different selenium status in rats author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Biological Research

Print version ISSN 0716-9760

Abstract

ALEXANDRINO BECKER, TÂNIA CRISTINA  and  ALVES DE CASTRO-PRADO, MARIALBA A. Parasexuality in asexual development mutants of Aspergillus nidulans. Biol. Res. [online]. 2006, vol.39, n.2, pp.297-305. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602006000200012.

The parasexual cycle with parameiosis has been characterized previously by the occurrence of genetic recombination and haploidization inside heterokaryotic hyphae prior to conidial formation. The aim of current research was to characterize, through genetic and cytological analyses, an asexual development mutant strain of A. nidulans and to use it to obtain parameiotic segregants. Analyses showed the medusa phenotype of the B84 strain, whose mutant allele was mapped in the chromosome I. The heterokaryons B84(med)//G422(med+) and B84(med)//G839(brl) were formed in liquid MM+2% CM and inoculated in the appropriate selective media. Two mitotic segregant groups were obtained: aneuploids and haploid stable recombinants. Mitotic segregants, wild-types, and developmental mutants, which did not produce new visible mitotic sectors in the presence of Benomyl and which showed normal meiotic behavior during the sexual cycle, were classified as parameiotics

Keywords : parasexual cycle; conidiation mutants; mitotic recombination; haploid recombinants.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License