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Biological Research

Print version ISSN 0716-9760

Abstract

AVILA, ANA D et al. Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin. Biol. Res. [online]. 2007, vol.40, n.2, pp.173-183. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602007000200008.

Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptor

Keywords : Epidermal growth factor; epidermal growth factor receptor; hemolytic toxins; immunotoxins; monoclonal antibodies.

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