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Biological Research

Print version ISSN 0716-9760

Abstract

MORALES, GLAUCO; PAREDES, ADRIÁN; SIERRA, PATRICIA  and  LOYOLA, LUIS A. Antioxidant activity of 50% aqueous - ethanol extract from Acantholippia deserticola. Biol. Res. [online]. 2008, vol.41, n.2, pp.151-155. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602008000200004.

The antioxidant properties oí Acantholippia deserticola, a herb used in traditional northern Chilean medicine was studied using free radical - generating systems. The 50% aqueous - ethanol extract oí Acantholippia deserticola protected against non-enzymatic lipid peroxidation in microsomal membranes of rat, induced by an Fe++ - ascorbate system and measured spectrophotometrically by the TBARS test, and had strong free radical scavenging capacities on stable ABTS and DPPH radicáis. The results shows that the IC50 valué of the 50% aqueous - ethanolic extract oí A.deserticola is 18 ± 0.5 μg/mL in DPPH radical - scavenging, 15 ± 0.8 μg/mL in lipid peroxidation , Total Antioxidant Activity (TAA) is 0.95 mM of Trolox per mg/mL of extract. The total phenolics content of extract is 725 ± 12 mg of gallic acid equivalent per g of dried extract. The results indicate that the 50% aqueous - ethanol extract oí Acantholippia deserticola clearly has antioxidant properties.

Keywords : Acantholippia deserticola; northern Chilean medicinal plant; phenolics; free radicáis scavenging; microsomal lipid peroxidation.

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