SciELO - Scientific Electronic Library Online

vol.42 issue4Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytesMycophenolate mofetil versus Rapamycin in Han: SPRD rats with Polycystic Kidney Disease author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Biological Research

Print version ISSN 0716-9760


SOUZA VIANA, ELISETH et al. Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk. Biol. Res. [online]. 2009, vol.42, n.4, pp.427-436. ISSN 0716-9760.

The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy. The H. alvei 071 hall mutant was deficient in biofilm formation. All these results showed that the quorum sensing system is probably involved in the regulation of biofilm formation by H. alvei.

Keywords : acyl homoserines lactones; biofilm; furanones; quorum sensing.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License