Resumen

NIKOLIC, Ljiljana M et al. Effect of alternating the magnetic feld on phosphate metabolism in the nervous system of Helix pomatia. Biol. Res. [online]. 2010, vol.43, n.2, pp.243-250. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602010000200012.

The effect of extremely low frequency magnetic felds (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The infuence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by ³¹P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na⁺/K⁺-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and decreased enzyme activity, compared to the 7-day treatment group. Our results can be explained in terms of: 1. increase in phosphate turnover by exposure to the ELF-MF for the period of 7 days, and 2. adaptation of phosphate metabolism in the nervous system of snails to prolonged ELF-MF exposure.

Palabras clave : ELF-MF; garden snail; ³¹P NMR spectroscopy; phosphate turnover enzymes.

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