SciELO - Scientific Electronic Library Online

 
vol.44 issue4Slow axoplasmic transport under scrutinyPorcine oviduct sperm binding glycoprotein and its deleterious effect on sperm: a mechanism for negative selection of sperm? author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Biological Research

Print version ISSN 0716-9760

Abstract

OLGUIN, Hugo C. Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts. Biol. Res. [online]. 2011, vol.44, n.4, pp.323-327. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602011000400002.

The transcription factor Pax7 negatively regulates the activity of the muscle regulatory transcription factor MyoD, preventing muscle precursor cells from undergoing terminal differentiation. In this context, the ratio between Pax7 and MyoD protein levels is thought to be critical in allowing myogenesis to proceed or to maintain the undifferentiated muscle precursor state. We have previously shown that Pax7 is subject to rapid down regulation in differentiating myoblasts, via a proteasome-dependent pathway. Here we present evidence indicating that Pax7 is also subject to caspase-3-dependent regulation. Furthermore, simultaneous inhibition of caspase-3 and proteasome activity induced further accumulation of Pax7 protein in differentiating myoblasts. These results suggest that at early stages of muscle differentiation, Pax7 levels are regulated by at least two independent mechanisms involving caspase-3 and proteasome activity.

Keywords : Pax7; caspase-3; calpains; proteasome; satellite cells; muscle differentiation.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License