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Biological Research

Print version ISSN 0716-9760

Abstract

MAHESH, Ayyavu; THANGADURAI, Devarajan  and  MELCHIAS, Gabriel. Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze. Biol. Res. [online]. 2012, vol.45, n.2, pp.131-136. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602012000200004.

An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.

Keywords : Asteraceae; Launaea sarmentosa; Leaf explant; Organogenesis.

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