SciELO - Scientific Electronic Library Online

 
vol.50Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous miceRole of regenerating gene IA expression on local invasion and survival in nasopharyngeal carcinoma author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Biological Research

Print version ISSN 0716-9760

Abstract

DU, Maotao; ZHANG, Zhong  and  GAO, Tao. Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells. Biol. Res. [online]. 2017, vol.50, 36. ISSN 0716-9760.  http://dx.doi.org/10.1186/s40659-017-0141-8.

Background

Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells.

Methods

Melanoma cell lines WM266-4 and A2058 were treated either with or without piceatannol. Cell viability and cell apoptosis were assessed by using MTT and Annexin V/PI assay, respectively. Cells were transfected with specific miRNA using Lipfectamine 2000. miRNA bingding ability to 3'-UTR region within specific gene was assed by firefly luciferase analysis. Gene and protein expression was eveluated by qRT-PCR and western blot analysis, respectively.

Results

Our study showed that piceatannol inhibited WM266-4 and A2058 cells growth and induced apoptosis. Totally, 16 differentially expressed miRNAs were screened out including 8 up-regulated and 8 down-regulated miRNAs. Expression level of miR-181a is significantly higher in piceatannol-treated cells than normal control and is lower in melanoma cancer tissues than its adjacent normal tissues. Bcl-2 is a target gene of miR-181a. Moreover, silencing of miR-181a reverses the decrease of cell viability induced by piceatannol in WM266-4 and A2058 cells. Taken together, present study uncovered the ability of piceatannol to repress melanoma cell growth and clarified the contribution of miR-181a in the anticancer role of piceatannol.

Conclusion

The present study proposes that piceatannol can be taken into account to be a hopeful anticancer agent for melanoma.

Keywords : Piceatannol; Apoptosis; microRNA-181-a; Melanoma cells.

        · text in English     · English ( pdf )