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Biological Research

versión impresa ISSN 0716-9760

Resumen

XING, Feng; SONG, Zhijiao  y  HE, Yuanying. MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2. Biol. Res. [online]. 2018, vol.51, 50.  Epub 12-Jun-2019. ISSN 0716-9760.  http://dx.doi.org/10.1186/s40659-018-0199-y.

Background:

Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer.

Methods:

The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation.

Results:

Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3’-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo.

Conclusion:

Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2.

Palabras clave : Ovarian cancer; miR-219-5p; HMGA2; Growth; Metastasis.

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