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Biological Research

Print version ISSN 0716-9760

Abstract

GARCIA-GONZALES, Rolando et al. Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae). Biol. Res. [online]. 2018, vol.51, 42.  Epub Nov 28, 2018. ISSN 0716-9760.  http://dx.doi.org/10.1186/s40659-018-0193-4.

Background:

Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement.

Results:

Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively.

Conclusions:

From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

Keywords : Microsatellite; Pyrosequencing; Chilean berry; Gaultheria pumila.

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