SciELO - Scientific Electronic Library Online

 
vol.53Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cellsLncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Biological Research

Print version ISSN 0716-9760

Abstract

GARCIA, Patricia et al. Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor. Biol. Res. [online]. 2020, vol.53, 13.  Epub Apr 24, 2020. ISSN 0716-9760.  http://dx.doi.org/10.1186/s40659-020-00282-7.

Background:

Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry.

Results:

After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice.

Conclusions:

The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.

Keywords : Gallbladder cancer; Cancer cell lines; Ascites; Native American ancestry; Gene expression profile.

        · text in English     · English ( pdf )