Abstract

VASQUEZ, Bélgica. CBG PRESENCE IN THE OVARIC STROMA OF THE MAMMALS. Rev. chil. anat. [online]. 2001, vol.19, n.3, pp.279-284. ISSN 0716-9868.  http://dx.doi.org/10.4067/S0716-98682001000300008.

The plasma corticoid binding globulin (CBG), has the capability to irreversible fixes steroid hormones (cortisone, corticosterone and progesterone). This molecule, acts directly on  the steroid transports,  changing their blood concentration and  influencing the hormonal action. The synthesis occurs mainly in the liver, but also in other organs and tissues such as the kidney, pituitary, prostate, endometrium and placenta. The objective of this study was to trace the presence of CBG on the ovarian stroma of the cow, bitch, cat and rat under a morphological point of view using an inmunocytochemical (ICQ) method. The ovarian samples were fixed in methacarn and embedding in paraplast. Serial cuts were performed to each sample and  the ICQ was done using the PAP method and an specific  polyclonal CBG human antibody was used. The results showed a selective immune positive reaction to CBG on the cummulus oophorus and granulosa cells.  In addition, some other types of cells found in the ovarian stroma in all the species studied showed a similar reaction. The immune reaction and the cell density varied according the state of the ovarian cycle. These cells were found in close contact with the blood vessels where a high interchange occurs. In conclusion, these results show that the CBG could be directly involved  in the reproductive process related to the  steroidal hormonal transports in the female reproductive system of these mammalian species

Keywords : CBG; Ovary; Immunocitochemistry; Bos taurus; Canis familiaris; Felix Catus; Ratus norvergicus.